A silver color metal and enamel device 1 1/8 inches (2.86 cm) in height overall, consisting of a horizontal silver stylized stretcher crossed by a red and silver stylized stretcher bearing a silver serpent entwined about a silver tent peg, the securing ring enclosing a blue disc charged with three silver mullets, two and one, all above a semi-circular maroon scroll pendant from the silver stretcher and inscribed "PRO VULNERATUS" in silver letters.
Maroon and white/silver are the colors traditionally associated with the U.S. Army Medical Corps. The stretchers form a cross emphasizing medical service and compassion. The stretchers and tent peg refer to the mission of the unit. The silver serpent entwined on the tent peg alludes to the staff of Aesculapius. The blue disc and silver stars are adapted from the Tennessee State flag and denote the unit's location in the Tennessee Army National Guard. The translation of the motto is "For the Wounded."
The distinctive unit insignia was originally approved for the 300th Mobile Army Surgical Hospital on 12 December 1988. It was redesignated effective 1 September 1992, with description revised, for the 300th Combat Support Hospital, Tennessee Army National Guard.